저출력 초음파와 기계적 장력이 MG-56 세포의 골모세포 분화에 미치는 영향
Potential Effect of Low Intensity Pulsed Ultrasound and Mechanical Tensile Strain on Osteoblastic differentiation of MG-65 Cells
최용식, 금동호, 황대석, 유미현, 김규천, 김욱규,
소속 상세정보
최용식 ( Choi Yong-Sik ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
금동호 ( Geum Dong-Ho ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
황대석 ( Hwang Dae-Seok ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
유미현 ( Ryu Mi-Heon ) - 부산대학교 치의학전문대학원 구강병리학교실
김규천 ( Kim Gyoo-Cheon ) - 부산대학교 치의학전문대학원 구강해부학교실
김욱규 ( Kim Uk-Kyu ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
Abstract
Tissue engineering has been rapidly developed in oral and maxillofacial reconstruction. Biocompatible scaffold from chemically composites seeded with stem cells is essential and several growth factors for bone formation and angiogenesis are also required. To overcome limited activity of new bone formation with scaffolds, several biomechanical stimulation methods on cells have been made to grow cells in scaffold. Several bioreactors have been developed for real tissue growth in culture laboratory. In addition to biological stimulants like BMP, growth factors and exogenous drugs, biomechanical stimulation technique has also been known as an effective method in cell differentiation. We developed our own bioreactor with tensile mechanical strains. Then we tested with it for detection of suitable biomechanical effect on the cell differentiation and proliferation. And we also compared the results with the effect of low intensity pulsed ultrasound (LIPUS). Mechanical strain group showed more rapid reaction with cell differentiation and proliferation than non-mechanical strain group. Mechanical strain groups stimulated with 0.5∼0.7Hz for 6 hours and 8 hours showed more active cell differentiation than the group with 0.5∼0.7Hz for 2.5 hours tensile strain stimulation. Group of LIPUS also showed more rapid reaction in cell differentiation and proliferation. LIPUS with 3MHz showed more cell reaction than the LIPUS group with 1MHz. Our results showed the positive effect on differentiation and proliferation of cell with mechanical tensile strain, LIPUS both.
키워드
Osteogenic activity; Tensile strain; Low intensity pulsed ultrasound
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